Gourt > Science > Biology > Cell Biology > Methods and Techniques > Cell Culture
Cell culture is the process by which either prokaryotic or eukaryotic cells are grown under controlled conditions. In practice the term "cell culture" has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture.
Animal cell culture became a routine laboratory technique in the 1950s, but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th Century.
History
19th Century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body.* In 1885 Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907-1910, establishing the methodology of tissue culture. Schiff, Judith Ann. Yale Alumni Magazine, February 2002. More on [ Cell culture ]
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New Stem Cell Lines On the Way [Dispatches from the Culture Wars]: Here's one of the major changes that Obama has b... http://bit.ly/92rtEl
nicedoggy (David Beckow) Sun, 06 Dec 2009 14:26:10 -0000
New Stem Cell Lines On the Way [Dispatches from the Culture Wars]: Here's one of the major changes that Obama has b... http://bit.ly/92rtEl
Senior Director/Director  Antibody Production and Mammalian Cell Culture Job
HotJobSearch (Job Seeker) Sun, 06 Dec 2009 14:20:21 -0000
Senior Director/Director Antibody Production and Mammalian Cell Culture Job
Manufacturing Technologist (cell culture, viral propagation) http://ff.im/-cv37D
ManufacturingWk (Manufacturing Jobs) Sun, 06 Dec 2009 04:33:31 -0000
Manufacturing Technologist (cell culture, viral propagation) http://ff.im/-cv37D
Novartis Inaugurates Flu Cell-Culture Vaccine Facility in North Carolina http://tinyurl.com/yj24aty #h1n1 #de #dover
SwineFluNewsUSA (Ken Wright) Sat, 05 Dec 2009 23:03:19 -0000
Novartis Inaugurates Flu Cell-Culture Vaccine Facility in North Carolina http://tinyurl.com/yj24aty #h1n1 #de #dover
"You are an alien archaeologist. Excavations reveal detergent bottle, cell phone, Xmas lights, McD's menu. Explain U.S. culture." #evilexam
csammells (Clare Sammells) Sat, 05 Dec 2009 20:37:36 -0000
"You are an alien archaeologist. Excavations reveal detergent bottle, cell phone, Xmas lights, McD's menu. Explain U.S. culture." #evilexam
Process Development Engineer - Cell Culture http://bit.ly/5Dzt7j
jobsineng (Engineering Jobs) Sat, 05 Dec 2009 17:18:36 -0000
Process Development Engineer - Cell Culture http://bit.ly/5Dzt7j
Bart's Cookbook and Lab Protocols - Introduction to the steps and reagents involved in tissue culture, at the Sefton Lab, Salk Institute, San Diego, California.
Cell Biology Lab - Tissue Culture Methods - Introduction to methodology for growing and maintaining tissues and cells. Links to descriptions of specific cell lines, resource for suppliers and related links, maintained by the University of New South Wales School of Anatomy.
Meta Description: [ The School of Medical Sciences is the largest School within the Faculty of Medicine at UNSW, Sydney, Australia. The School teaches courses in the Undergraduate Medicine Program as well as a wide range of other Science and Medicine programs at the undergraduate and postgraduate level. ]
Cell Culture Chat - Bulletin board for the discussion of cell line and methodology, maintained by an instrument distributor Spectrum Laboratories, Inc., Rancho Dominguez, California.
Meta Description: [ Ask questions and get answers to all of your cell culture questions. ]
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Endothelial Consult - Advise on vascular and lymphatic endothelial cell cultures in vitro.
Plant Tissue Culture - Laboratory protocol for micropropagation from stem tip, node, meristem, embryo or seed. Includes forum maintained by the National Health Museum, Washington, DC.
Tissue Culture Methods - Describes the equipment and work space requirements for cell and tissue growth. Includes details on cryopreservation, maintenance, media prep procedures, safety considerations and link to the masters degree at University of Maryland Baltimore County.
Worthington Tissue Dissociation Guide - Resource on cell isolation theory and techniques, including use-tested systems, FAQ, and annotated references searchable by multiple criteria from Lakewood, NJ.
Meta Description: [ technical reference for primary cell isolation ]
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