Gourt > Science > Biology > Cell Biology > Methods and Techniques > Cell Culture
Cell culture is the process by which either prokaryotic or eukaryotic cells are grown under controlled conditions. In practice the term "cell culture" has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture.
Animal cell culture became a routine laboratory technique in the 1950s, but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th Century.
History
19th Century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body.* In 1885 Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907-1910, establishing the methodology of tissue culture. Schiff, Judith Ann. Yale Alumni Magazine, February 2002. More on [ Cell culture ]
Cryobiology :: Biology
Gene Therapy :: Human
Living Systems :: Products and Services
Tissue Engineering :: Biomedical Engineering
Why do I love Soft Cell and Culture Club so much? So much.
ginaaaa (Gina Richards) Sat, 07 Nov 2009 07:34:22 -0000
Why do I love Soft Cell and Culture Club so much? So much.
#Novartis' cell culture-based flu #vaccine Celtura gets German approval - pharmabiz.com : http://bit.ly/2IQe50
live_h1n1 (Live H1N1) Sat, 07 Nov 2009 07:29:58 -0000
#Novartis' cell culture-based flu #vaccine Celtura gets German approval - pharmabiz.com : http://bit.ly/2IQe50
So what, I know I'm a N.E.R.D. when it comes to cell phones, Japanese culture, and stuff. I'm still a skateboarder/role model to alot!
djrisingsun (Brando Bennett) Sat, 07 Nov 2009 03:06:34 -0000
So what, I know I'm a N.E.R.D. when it comes to cell phones, Japanese culture, and stuff. I'm still a skateboarder/role model to alot!
í´! ì¸ë¯¸ë ë°í ëë¬ë¤. ë물 cell culture systemì´ ì무리 í¸íë¤ íëë¼ë 기본ì ì¸ ì ì íì ì´í´í ì¤ ììëë°. Double mutantì genetic epistasis를 ì¤ëª íë ìê° 1/3ì ë©ë리기 ììíëêµ°. ë°ë³´ë¤!
dh_park (donha park) Sat, 07 Nov 2009 02:35:08 -0000
휴! 세미나 발표 끝났다. 동물 cell culture system이 아무리 편하다 하더라도 기본적인 유전학은 이해할 줄 알았는데. Double mutant와 genetic epistasis를 설명하는 순간 1/3은 멍때리기 시작하더군. 바보들!
RT @Bionity_COM: Novartis receives regulatory approval for cell culture-based Influenza A(H1N1) pandemic vaccine http://bit.ly/3iEPWD
ModelN (Model N) Fri, 06 Nov 2009 18:50:45 -0000
RT @Bionity_COM: Novartis receives regulatory approval for cell culture-based Influenza A(H1N1) pandemic vaccine http://bit.ly/3iEPWD
Biosecurity News: H1N1 vax; Antivirals; Mask efficacy; Cell culture vax; Surveillance; Flu in animals; Dengue; Anthrax. http://tiny.cc/bnib
BiosecurityCntr (Cntr for Biosecurity) Fri, 06 Nov 2009 18:49:37 -0000
Biosecurity News: H1N1 vax; Antivirals; Mask efficacy; Cell culture vax; Surveillance; Flu in animals; Dengue; Anthrax. http://tiny.cc/bnib
Bart's Cookbook and Lab Protocols - Introduction to the steps and reagents involved in tissue culture, at the Sefton Lab, Salk Institute, San Diego, California.
Cell Biology Lab - Tissue Culture Methods - Introduction to methodology for growing and maintaining tissues and cells. Links to descriptions of specific cell lines, resource for suppliers and related links, maintained by the University of New South Wales School of Anatomy.
Meta Description: [ The School of Medical Sciences is the largest School within the Faculty of Medicine at UNSW, Sydney, Australia. The School teaches courses in the Undergraduate Medicine Program as well as a wide range of other Science and Medicine programs at the undergraduate and postgraduate level. ]
Cell Culture Chat - Bulletin board for the discussion of cell line and methodology, maintained by an instrument distributor Spectrum Laboratories, Inc., Rancho Dominguez, California.
Meta Description: [ Ask questions and get answers to all of your cell culture questions. ]
500
Endothelial Consult - Advise on vascular and lymphatic endothelial cell cultures in vitro.
Plant Tissue Culture - Laboratory protocol for micropropagation from stem tip, node, meristem, embryo or seed. Includes forum maintained by the National Health Museum, Washington, DC.
Tissue Culture Methods - Describes the equipment and work space requirements for cell and tissue growth. Includes details on cryopreservation, maintenance, media prep procedures, safety considerations and link to the masters degree at University of Maryland Baltimore County.
Worthington Tissue Dissociation Guide - Resource on cell isolation theory and techniques, including use-tested systems, FAQ, and annotated references searchable by multiple criteria from Lakewood, NJ.
Meta Description: [ technical reference for primary cell isolation ]
| Cell culture | |
| Next Video | |
