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Enzyme kinetics is the study of the rates of chemical reactions catalyzed by enzymes. The study of enzyme kinetics provides information on how enzymes work, how their activity is controlled in the body and how drugs and poisons inhibit their reactions.

Enzymes are molecular machines that manipulate other molecules. The information provided by an enzyme's structure is akin to a complete blueprint of a machine. However, the information provided by kinetic studies is similar to a movie of this machine in action. In order to completely understand how enzymes work, we need to know both their structures and their mechanisms.

Kinetic studies on enzymes that only bind one substrate, such as catalase, aim to measure the affinity with which the enzyme binds this substrate and how fast it can turn it into a product. When enzymes bind multiple substrates, enzyme kinetics can also show the order in which these substrates bind and the order in which products are released.

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Proteins and Enzymes :: Biomolecules

 
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Advances in Enzyme Regulation - Reports progress at the cutting edge in the strategic area of regulation at the molecular level.

Binding Database - Resource for deposition of measured binding affinities for natural, genetically or chemically modified, and synthetic compounds by isothermal titration calorimetry (ITC) and other inhibition methods. Maintained at UMBI, Rockville, MD.

404 Enzyme Biochemistry Chapter - A chapter in the MIT Biology Hypertextbook site. Provides a more in-depth look at enzyme reactions including enzyme inhibition and the different modes of inhibition.

Enzymes - Provides an introduction, for the uninitiated, to enzymes and how they function.

Thermodynamics of Enzyme Catalyzed Reactions - Database of thermodynamic parameters of enzyme catalyzed reactions.

University of Murcia, Spain - GEnz - Scientific research on enzyme kinetics and regulation. Technological development of enzymology and applications.

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Pre-steady state enzyme kinetics via digital microfluidics (droplets) and MALDI-TOF MS ...
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